pAAV-hsyn-NE2h
(Plasmid #208687)

• Purpose: Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons
• Depositing Lab: Yulong Li
• Publication: Monitoring norepinephrine release in vivo using next-generation GRABNE sensors (unpublished)

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4495
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
• Alt name: GRAB_NE2h
• Alt name: NE2h
• Species: H. sapiens (human)
• Insert Size (bp): 1992
• Promoter: hSyn

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACGGGCGCGACCATCTGCGC
• 3′ sequencing primer: CATTAAAGCAGCGTATCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.22.546075v1 for bioRxiv preprint.