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Addgene

pIOM14
(Plasmid #20868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKAS32
  • Backbone manufacturer
    Karen Skorupski and Ronald K. Taylor
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Bacterial Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Strains producing the π protein from λ phage. Ej S17λpir, CC118λpir.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    aphA::aacC1
  • Alt name
    kanamycin resistance gene interrupted by gentamycin resistance gene
  • Species
    bacteria
  • Insert Size (bp)
    3700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains aacC1 which confers resistance to gentamycin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIOM14 was a gift from Carmen Beuzón (Addgene plasmid # 20868 ; http://n2t.net/addgene:20868 ; RRID:Addgene_20868)

  • For your References section:

    Suicide vectors for antibiotic marker exchange and rapid generation of multiple knockout mutants by allelic exchange in Gram-negative bacteria. Ortiz-Martin I, Macho AP, Lambersten L, Ramos C, Beuzon CR. J Microbiol Methods. 2006 Dec . 67(3):395-407. 10.1016/j.mimet.2006.04.011 PubMed 16750581
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