pEGFP-Kif2c
(Plasmid
#208651)
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PurposeMammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208651 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markerseGFP
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKif2c
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Alt nameMCAK
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SpeciesH. sapiens (human)
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GenBank IDNM_006845.4
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Entrez GeneKIF2C (a.k.a. CT139, KNSL6, MCAK)
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byLinda Wordeman
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-Kif2c was a gift from Samuel Bakhoum (Addgene plasmid # 208651 ; http://n2t.net/addgene:208651 ; RRID:Addgene_208651) -
For your References section:
Non-cell-autonomous cancer progression from chromosomal instability. Li J, Hubisz MJ, Earlie EM, Duran MA, Hong C, Varela AA, Lettera E, Deyell M, Tavora B, Havel JJ, Phyu SM, Amin AD, Budre K, Kamiya E, Cavallo JA, Garris C, Powell S, Reis-Filho JS, Wen H, Bettigole S, Khan AJ, Izar B, Parkes EE, Laughney AM, Bakhoum SF. Nature. 2023 Aug;620(7976):1080-1088. doi: 10.1038/s41586-023-06464-z. Epub 2023 Aug 23. 10.1038/s41586-023-06464-z PubMed 37612508