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Addgene

pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56
(Plasmid #208645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC-ODN
  • Modifications to backbone
    refer to (Addgene#208642)
  • Vector type
    CRISPR ; ssDNA donnor preparation for knock-in of RPL3 C-ter tag

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Twin Strep tag with RPL3 genome HomoArm
  • Alt name
    Donnor DNA for CRISPR tagging of RPL3
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    Two SNP (rs749555789, rs760194863) were introduced to abolish the PAM of sgRNA in (Addgene#208647 and #208979)
  • GenBank ID
    5'HomoArm: NC_000022.11:39313122~39312943 3'HomoArm: NC_000022.11:39312939~39312773
  • Entrez Gene
    RPL3 (a.k.a. ASC-1, L3, TARBP-B, uL3)
  • Tag / Fusion Protein
    • Twin Strep

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56 was a gift from Quanfu Ma (Addgene plasmid # 208645 ; http://n2t.net/addgene:208645 ; RRID:Addgene_208645)
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