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Addgene

pUC-ODN-hsRPL3-CterTwStrep
(Plasmid #208644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC-ODN
  • Modifications to backbone
    refer to (Addgene#208642)
  • Vector type
    CRISPR ; ssDNA donnor preparation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Twin Strep tag with RPL3 genome HomoArm
  • Alt name
    Donnor DNA
  • Species
    H. sapiens (human), Synthetic
  • GenBank ID
    5'HomoArm: NC_000022.11:39313122~39312943 3'HomoArm: NC_000022.11:39312939~39312773
  • Entrez Gene
    RPL3 (a.k.a. ASC-1, L3, TARBP-B, uL3)
  • Tag / Fusion Protein
    • Twin Strep

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

#important# This is a Intermediate plasmid, and WILL fail the CRISPR knock-in. Use Addgene#208645, do not use this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-ODN-hsRPL3-CterTwStrep was a gift from Quanfu Ma (Addgene plasmid # 208644 ; http://n2t.net/addgene:208644 ; RRID:Addgene_208644)
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