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Addgene

pUC-ODN2
(Plasmid #208643)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208643 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Modifications to backbone
    Remove BspQI (690), BsrDI (1760, 1934), BtsI (592, 2099, 2119) sites by point mutagenesis, remove BsaI (1760) site by point mutagenesis, remove BsmBI site by deletion of 2623~(origin)~85.
  • Vector type
    Long single strand DNA production

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    point mutations that remove BspQI, BsrDI, BtsI were adoptted from Nagahora Hitoshi's work (genbank MA009870), point mutations that remove BsaI was adoptted from Wei Li's work (addgene#121957).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that this is only a pUC19 removed indicated intrisic enzyme site, BspQI or BsrDI needed for ssDNA preparation need to be introduced together with the inserts. Method used was as discribed in "https://www.neb.com/-/media/nebus/files/application-notes/appnote_improved_methods_for_sdm_using_nebuilder_hifi_dna_assembly_master_mix.pdf"

>mutagenesis_primers

pUC-ΔBts592-F: 5' CGTTGCGCTCACAGCCCGCTTTCCAG 3'
pUC-ΔBts592-R: 5' CTGGAAAGCGGGCTGTGAGCGCAACG 3'

pUC-ΔBspQ690-F: 5' GTATTGGGCGCTGTTCCGCTTCCTC 3'
pUC-ΔBspQ690-R: 5' GAGGAAGCGGAACAGCGCCCAATAC 3'

pUC-ΔBsrD1760-F: 5' CAGTGCTGCAATAATACCGCGAGACC 3'
pUC-ΔBsrD1760-R: 5' GGTCTCGCGGTATTATTGCAGCACTG 3'

pUC-ΔBsrD1934-F: 5' CGTTGTTGCCATAGCTACAGGCATCGTG 3'
pUC-ΔBsrD1934-R: 5' CACGATGCCTGTAGCTATGGCAACAACG 3'

pUC-ΔBts2099&2119-F: 5' CCGCTGTGTTATCACTCATGGTTATGGCAGCACTACATAATTCTC 3'
pUC-ΔBts2099&2119-R: 5' TATGTAGTGCTGCCATAACCATGAGTGATAACACAGCGGCCAAC 3'

pUC-ΔBsa1766-F: 5' CAATAATACCGCGAGAGCCACGCTCACC 3'
pUC-ΔBsa1766-R: 5' GGTGAGCGTGGCTCTCGCGGTATTATTG 3'

pUC-Δ2623~85-F: 5' GAAAAGTGCCACCTGACGTCGAGCAGACAAGCCC 3'
pUC-Δ2623~85-R: 5' GGGCTTGTCTGCTCGACGTCAGGTGGCACTTTTC 3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-ODN2 was a gift from Quanfu Ma (Addgene plasmid # 208643 ; http://n2t.net/addgene:208643 ; RRID:Addgene_208643)