pUC-T7-hTP53-P0~670-FLuc
(Plasmid #208640)

• Purpose: for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
• Depositing Lab: Quanfu Ma
• Publication: RBM24 interacts with RPL3 and regulates RPL3-eIF6 interaction (unpublished)

Backbone

• Vector backbone: pUC19
• Vector type: Luciferase ; In vitor transcription template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TP53, UTR and partial CDS
• Alt name: P53-IRES-P0
• Species: H. sapiens (human)
• Mutation: UTR and partial CDS in-frame with F-Luc
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
• Promoter: T7
• Tags / Fusion Proteins:
  • T7 promoter (N terminal on insert)
  • Firefly Luciferase (F-Luc) (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13-F
• 3′ sequencing primer: M13-R

Resource Information

• Supplemental Documents:
  • pUC-T7-hTP53-P0~670-FLuc
• A portion of this plasmid was derived from a plasmid made by: F-luc was cloned from pGL3-Basic.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For in vitro transcription, Linearize with HindIII