pUC-T7-J1~398-Luc
(Plasmid #208639)

• Purpose: for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
• Depositing Lab: Quanfu Ma
• Publication: RBM24 interacts with RPL3 and regulates RPL3-eIF6 interaction (unpublished)

Backbone

• Vector backbone: pUC19
• Vector type: Luciferase ; In vitro transcription template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
• Alt name: HCV-IRES
• Alt name: Internal Ribosome Entry Site
• Alt name: HCV-5'UTR
• Species: Hepatitis C virus
• Mutation: nt 1~398, in frame with donstream F-Luc
• GenBank ID: AB047639
• Promoter: T7
• Tags / Fusion Proteins:
  • T7 promoter (N terminal on insert)
  • Firefly Luciferase (F-Luc (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13-F
• 3′ sequencing primer: M13-R

Resource Information

• Supplemental Documents:
  • pUC-T7_J1~398_Luc.gb
• A portion of this plasmid was derived from a plasmid made by: JFH1 is a generous gift from Prof. Wakita Takaji. F-luc was cloned from pGL3-Basic.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For in vitro transcription, Linearize with XbaI