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Addgene

pET28-eIF4E-TwStrep
(Plasmid #208638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208638 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-3'TwStrep
  • Modifications to backbone
    refer to (Addgene#208053)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    we use BL21 (DE3) for expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human eIF4E (ENST00000450253)
  • Species
    H. sapiens (human)
  • GenBank ID
    ENST00000450253
  • Entrez Gene
    EIF4E (a.k.a. AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E)
  • Promoter T7
  • Tags / Fusion Proteins
    • Twin Strep (C terminal on backbone)
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pETupstream, T7p
  • 3′ sequencing primer T7ter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-eIF4E-TwStrep was a gift from Quanfu Ma (Addgene plasmid # 208638 ; http://n2t.net/addgene:208638 ; RRID:Addgene_208638)
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