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pET28-H12TEV-hEIF5B-587~1220
(Plasmid #208637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28-H12TEV
  • Modifications to backbone
    refer to (Addgene#208635)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    we use BL21 (DE3) for expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human eIF5B (ENST00000289371) aa 587~1220
  • Alt name
    eIF5B
  • Species
    H. sapiens (human)
  • Mutation
    contain SNP rs1376443, protein sequence not changed, only aa 587~1220, other residues not included
  • GenBank ID
    ENST00000289371
  • Entrez Gene
    EIF5B (a.k.a. IF2)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xhis (N terminal on backbone)
    • 6xhis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pETupstream, T7p
  • 3′ sequencing primer T7ter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tag: 6xhis-thrombin-6xhis-TEV, not continuous 12x his

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-H12TEV-hEIF5B-587~1220 was a gift from Quanfu Ma (Addgene plasmid # 208637 ; http://n2t.net/addgene:208637 ; RRID:Addgene_208637)
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