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Addgene

pET28-Flag-RBM24-95~236
(Plasmid #208634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208634 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    Merck-Millipore (Novagen)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human RBM24 (ENST00000216146) aa 95~236
  • Alt name
    RBM24
  • Species
    H. sapiens (human)
  • Mutation
    only aa 95~236, other residues not included
  • GenBank ID
    ENST00000379052
  • Entrez Gene
    RBM24 (a.k.a. RNPC6, dJ259A10.1)
  • Promoter T7
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

#important# For this plasmid, we failed to obtain enough soluble protein with standard protocol using BL21(DE3) and Ni-NTA. Need further optimization.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-Flag-RBM24-95~236 was a gift from Quanfu Ma (Addgene plasmid # 208634 ; http://n2t.net/addgene:208634 ; RRID:Addgene_208634)
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