pET28-5'TwStrep
(Plasmid #208620)

• Purpose: (Empty Backbone) Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
• Depositing Lab: Quanfu Ma
• Publication: RBM24 interacts with RPL3 and regulates RPL3-eIF6 interaction (unpublished)

Backbone

• Vector backbone: pET28a(+)
• Backbone manufacturer: Merck-Millipore (Novagen)
• Modifications to backbone: add Twin Strep tag
• Vector type: Bacterial Expression
• Promoter: T7
• Tag / Fusion Protein:
  • Twin Strep (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: we use BL21 (DE3) for expression
• Copy number: Low Copy

Resource Information

• Supplemental Documents:
  • pET28-5'TwStrep.gb
• A portion of this plasmid was derived from a plasmid made by: Strep tag is ® of IBA lifescience.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We modified Twin Strep, reduced repeat, for better seamless cloning, the nucleic acids are different from that of IBA lifescience, amino acids are the same.