HT152_pAAV_EF1a_fDiO_pAce
(Plasmid
#208610)
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PurposeExpressing pAce in a Flp-dependent manner
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208610 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAce
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SpeciesSynthetic
- Promoter EF1a
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Tag
/ Fusion Protein
- mNeon Green
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a_F
- 3′ sequencing primer WPRE_R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byVincent A. Pieribone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HT152_pAAV_EF1a_fDiO_pAce was a gift from Adam Cohen (Addgene plasmid # 208610 ; http://n2t.net/addgene:208610 ; RRID:Addgene_208610)
