pDisplay-AP-CFP-TM
(Plasmid #20861)

• Depositing Lab: Alice Ting
• Publication: Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7583-8.

Backbone

• Vector backbone: pDisplay
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AP-CFP-TM
• Species: E. Coli
• Insert Size (bp): 1092
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • CFP (C terminal on insert)
  • myc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please email with intended use and/or targets for this plasmid