pET21a-Streptavidin-Dead
(Plasmid #20859)

• Purpose: Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
• Depositing Lab: Alice Ting
• Publication: Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Backbone

• Vector backbone: pET21a
• Backbone size w/o insert (bp): 5443
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Streptavidin-Dead
• Species: E. Coli
• Insert Size (bp): 411
• Mutation: Triple mutant of wt Streptavidin N23A,S27D,S45A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please email with intended use and/or targets for this plasmid.