pLV-REPORT(PGK/CMV)-GtwyA
(Plasmid #208574)

• Purpose: LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin
• Depositing Lab: David Bersten
• Publication: Allen et al bioRxiv 2023.11.21.568191

Backbone

• Vector backbone: pLV
• Backbone manufacturer: inhouse
• Vector type: Mammalian Expression, Lentiviral, Synthetic Biology
• Selectable markers: Neomycin (select with G418), Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mnucTomato
• Species: Synthetic
• Promoter: minP

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: HSVtk
• Promoter: minP

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Gene/Insert 3

• Gene/Insert name: Neomycin
• Promoter: minP

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Gene/Insert 4

• Gene/Insert name: d2nucEGFP
• Species: Synthetic
• Promoter: PGK/CMV

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pLV-REPORT (CMV PGK)-GtwyA-scp1 new xhoi xbai.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry