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pLV-REPORT(PGKCMV)-nucTomato-hygro
(Plasmid #208573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone manufacturer
    inhouse
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mnucTomato
  • Species
    Synthetic
  • Promoter minP

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    d2nucEGFP
  • Species
    Synthetic
  • Promoter PGK/CMV

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    HygroR
  • Alt name
    Hygromycin resistance gene
  • Promoter PGK/CMV

Cloning Information for Gene/Insert 3

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Bersten Lab uses DH5alpha cells for transformation. Plasmid can be grown in NEB Stable but this is not required as this Lenti backbone is observed to be very stable.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-REPORT(PGKCMV)-nucTomato-hygro was a gift from David Bersten (Addgene plasmid # 208573 ; http://n2t.net/addgene:208573 ; RRID:Addgene_208573)

  • For your References section:

    dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. bioRxiv 2023.11.21.568191 10.1101/2023.11.21.568191
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