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Addgene

pCX:B3-α4ΔICD
(Plasmid #208513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCX
  • Backbone size w/o insert (bp) 4778
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcdha4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2967
  • Entrez Gene
    Pcdha4 (a.k.a. Cnr1, Crnr1)
  • Promoter Chicken β-actin
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CTTCTGGCGTGTGACCGG
  • 3′ sequencing primer CCATATGTCCTTCCGAGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCX:B3-α4ΔICD was a gift from Takeharu Nagai (Addgene plasmid # 208513 ; http://n2t.net/addgene:208513 ; RRID:Addgene_208513)

  • For your References section:

    Visualization of trans-interactions of a protocadherin-alpha between processes originating from single neurons. Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T. iScience. 2023 Jul 17;26(7):107238. doi: 10.1016/j.isci.2023.107238. eCollection 2023 Jul 21. 10.1016/j.isci.2023.107238 PubMed 37534169
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