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CN2039-rAAV-3xSP10ins-eHGT_354h-minRho-SYFP2-WPRE3-BGHpA
(Plasmid #208401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    rAAV
  • Backbone manufacturer
    custom
  • Backbone size w/o insert (bp) 4368
  • Total vector size (bp) 5088
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SYFP2
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter eHGT_354h (enhancer)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    SYFP2 insert was derived from Addgene plasmid#22905
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Allen Institute plasmid ALIAS: AiP12039
This work supported by NIH BRAIN Initiative grant UF1MH128339

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CN2039-rAAV-3xSP10ins-eHGT_354h-minRho-SYFP2-WPRE3-BGHpA was a gift from The Allen Institute for Brain Science & Jonathan Ting (Addgene plasmid # 208401 ; http://n2t.net/addgene:208401 ; RRID:Addgene_208401)

  • For your References section:

    Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex. Lee BR, Dalley R, Miller JA, Chartrand T, Close J, Mann R, Mukora A, Ng L, Alfiler L, Baker K, Bertagnolli D, Brouner K, Casper T, Csajbok E, Donadio N, Driessens SLW, Egdorf T, Enstrom R, Galakhova AA, Gary A, Gelfand E, Goldy J, Hadley K, Heistek TS, Hill D, Hou WH, Johansen N, Jorstad N, Kim L, Kocsis AK, Kruse L, Kunst M, Leon G, Long B, Mallory M, Maxwell M, McGraw M, McMillen D, Melief EJ, Molnar G, Mortrud MT, Newman D, Nyhus J, Opitz-Araya X, Ozsvar A, Pham T, Pom A, Potekhina L, Rajanbabu R, Ruiz A, Sunkin SM, Szots I, Taskin N, Thyagarajan B, Tieu M, Trinh J, Vargas S, Vumbaco D, Waleboer F, Walling-Bell S, Weed N, Williams G, Wilson J, Yao S, Zhou T, Barzo P, Bakken T, Cobbs C, Dee N, Ellenbogen RG, Esposito L, Ferreira M, Gouwens NW, Grannan B, Gwinn RP, Hauptman JS, Hodge R, Jarsky T, Keene CD, Ko AL, Korshoej AR, Levi BP, Meier K, Ojemann JG, Patel A, Ruzevick J, Silbergeld DL, Smith K, Sorensen JC, Waters J, Zeng H, Berg J, Capogna M, Goriounova NA, Kalmbach B, de Kock CPJ, Mansvelder HD, Sorensen SA, Tamas G, Lein ES, Ting JT. Science. 2023 Oct 13;382(6667):eadf6484. doi: 10.1126/science.adf6484. Epub 2023 Oct 13. 10.1126/science.adf6484 PubMed 37824669
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