PB-TA-ERP2-hcGAS(R255A)
(Plasmid
#208397)
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PurposeTo generate stable cell line expressing human cGAS(R255A) using PiggyBac transposon system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208397 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePB-TA-ERP2
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Vector typeMammalian Expression ; PiggyBac Transposon
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman cGAS
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SpeciesH. sapiens (human)
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MutationR255A
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Entrez GeneCGAS (a.k.a. C6orf150, D4, MB21D1, h-cGAS)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TA-ERP2-hcGAS(R255A) was a gift from Gaorav Gupta (Addgene plasmid # 208397 ; http://n2t.net/addgene:208397 ; RRID:Addgene_208397) -
For your References section:
MRE11 liberates cGAS from nucleosome sequestration during tumorigenesis. Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis JE, Gupta GP. Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6. 10.1038/s41586-023-06889-6 PubMed 38200309
