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Addgene

lentiMutB3GALT5-blast
(Plasmid #208381)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208381 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    lentiCas9-blast
  • Backbone manufacturer
    Addgene 52962
  • Backbone size w/o insert (bp) 8669
  • Total vector size (bp) 9719
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-1,3-galactosyltransferase 5
  • Alt name
    B3GALT5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    930
  • Mutation
    nucleotide change in PAM targeting sequence nt 515 g->a
  • GenBank ID
    NM_033173
  • Entrez Gene
    B3GALT5 (a.k.a. B3GalT-V, B3GalTx, B3T5, GLCT5, beta-1,3-GalTase 5, beta-3-Gx-T5, beta3Gal-T5)
  • Promoter EF-1 alpha core promoter
  • Tag / Fusion Protein
    • myc, FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer whole plasmid sequencing
  • 3′ sequencing primer whole plasmid sequencing
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Origene CAT#: RC211183

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.02.551727 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiMutB3GALT5-blast was a gift from Jennifer Kohler (Addgene plasmid # 208381 ; http://n2t.net/addgene:208381 ; RRID:Addgene_208381)