lentiMutB3GNT5-blast
(Plasmid #208380)

• Purpose: lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
• Depositing Lab: Jennifer Kohler
• Publication: Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxication (unpublished)

Backbone

• Vector backbone: lentiCas9-blast
• Backbone manufacturer: addgene 52962
• Backbone size w/o insert (bp): 8669
• Total vector size (bp): 9923
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Beta-1,3-N-Acetylglucosaminyltransferase 5
• Alt name: B3GNT5
• Species: H. sapiens (human)
• Insert Size (bp): 1134
• Mutation: nt 922 c->g (PAM site inactivation)
• GenBank ID: NM_032047
• Entrez Gene: B3GNT5 (a.k.a. B3GN-T5, beta3Gn-T5)
• Promoter: EF-1 alpha core promoter
• Tag / Fusion Protein:
  • myc, FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: whole plasmid sequencing
• 3′ sequencing primer: whole plasmid sequencing

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Origene NM_032047 (cat # RC206965)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.02.551727 for bioRxiv preprint.