pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein
(Plasmid
#208378)
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Purpose(Empty Backbone) Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208378 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTwist CMV
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Vector typeMammalian Expression
- Promoter human CMV
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Selectable markersPuromycin ; split puromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsCan be grown in DH5a or NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.03.574076 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein was a gift from Daniel Hawiger (Addgene plasmid # 208378 ; http://n2t.net/addgene:208378 ; RRID:Addgene_208378) -
For your References section:
Episomal Vectors for Stable Production of Recombinant Proteins and Engineered Antibodies. Fallahee I, Hawiger D. Antibodies (Basel). 2024 Mar 11;13(1):18. doi: 10.3390/antib13010018. 10.3390/antib13010018 PubMed 38534208
Map uploaded by the depositor.
