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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5
(Plasmid #208377)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist CMV
  • Backbone manufacturer
    Twist Bioscience
  • Backbone size (bp) 4304
  • Modifications to backbone
    Added 5621 bp of sequence
  • Vector type
    Mammalian Expression
  • Promoter human CMV
  • Selectable markers
    Puromycin ; EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Can be grown in DH5a or NEB Stable at 30-37˚C
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.03.574076 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5 was a gift from Daniel Hawiger (Addgene plasmid # 208377 ; http://n2t.net/addgene:208377 ; RRID:Addgene_208377)

  • For your References section:

    Episomal Vectors for Stable Production of Recombinant Proteins and Engineered Antibodies. Fallahee I, Hawiger D. Antibodies (Basel). 2024 Mar 11;13(1):18. doi: 10.3390/antib13010018. 10.3390/antib13010018 PubMed 38534208
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