pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5
(Plasmid
#208377)
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Purpose(Empty Backbone) Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTwist CMV
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Backbone manufacturerTwist Bioscience
- Backbone size (bp) 4304
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Modifications to backboneAdded 5621 bp of sequence
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Vector typeMammalian Expression
- Promoter human CMV
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Selectable markersPuromycin ; EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsCan be grown in DH5a or NEB Stable at 30-37˚C
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.03.574076 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5 was a gift from Daniel Hawiger (Addgene plasmid # 208377 ; http://n2t.net/addgene:208377 ; RRID:Addgene_208377) -
For your References section:
Episomal Vectors for Stable Production of Recombinant Proteins and Engineered Antibodies. Fallahee I, Hawiger D. Antibodies (Basel). 2024 Mar 11;13(1):18. doi: 10.3390/antib13010018. 10.3390/antib13010018 PubMed 38534208
Map uploaded by the depositor.
