Lenti-TO-Δ37 PPM1H-TMEM115-HA
(Plasmid
#208374)
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PurposeLentiviral expression of human Δ37-PPM1H with C-terminal TMEM115-HA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLenti-TO
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedelta37 PPM1H-TMEM115-HA
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SpeciesH. sapiens (human)
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Mutationdelta37
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Entrez GenePPM1H (a.k.a. ARHCL1, NERPP-2C, URCC2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-TO-Δ37 PPM1H-TMEM115-HA was a gift from Suzanne Pfeffer (Addgene plasmid # 208374 ; http://n2t.net/addgene:208374 ; RRID:Addgene_208374) -
For your References section:
Localization of PPM1H phosphatase tunes Parkinson's disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis. Wondwossen M Yeshaw , Ayan Adhikari, Claire Y Chiang, Herschel S Dhekne, Paulina S Wawro, Suzanne R Pfeffer. Proc Natl Acad Sci U S A. 2023 Oct 31;120(44) 10.1073/pnas.2315171120