SSH-pSUMO-α-COPI-WD40
(Plasmid
#208341)
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PurposeBacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameN-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
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Alt nameYeast coatomer αWD40 domain
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SpeciesS. pombe (fission yeast)
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MutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys
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Tags
/ Fusion Proteins
- Strep-tag (N terminal on backbone)
- His-tag (N terminal on backbone)
- SUMO-tag (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SSH-pSUMO-α-COPI-WD40 was a gift from Syed Saif Hasan (Addgene plasmid # 208341 ; http://n2t.net/addgene:208341 ; RRID:Addgene_208341) -
For your References section:
A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs. Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher T, Hasan SS. Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3. 10.1038/s41467-023-44076-3 PubMed 38102143
