pCMV-SpRYc-ABE8e-EGFP
(Plasmid
#208340)
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PurposeExpresses SpRYc-ABE8e in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208340 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV with a BR322 origin
- Total vector size (bp) 8244
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameecTadA(8e)-nSpRYc
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SpeciesSynthetic
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Insert Size (bp)4845
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MutationSc++ (1-1119), SpRY (1111-1368), D10A
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-Forward
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the sequence IDs for Plasmid 208336: pCAG-CBE4max-SpRYc-P2A-EGFP and Plasmid 208340: pCMV-SpRYc-ABE8e are incorrectly swapped in the associated paper. The correct plasmid ID for pCMV-SpRYc-ABE8e is 208340.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-SpRYc-ABE8e-EGFP was a gift from Pranam Chatterjee (Addgene plasmid # 208340 ; http://n2t.net/addgene:208340 ; RRID:Addgene_208340) -
For your References section:
PAM-flexible genome editing with an engineered chimeric Cas9. Zhao L, Koseki SRT, Silverstein RA, Amrani N, Peng C, Kramme C, Savic N, Pacesa M, Rodriguez TC, Stan T, Tysinger E, Hong L, Yudistyra V, Ponnapati MR, Jacobson JM, Church GM, Jakimo N, Truant R, Jinek M, Kleinstiver BP, Sontheimer EJ, Chatterjee P. Nat Commun. 2023 Oct 4;14(1):6175. doi: 10.1038/s41467-023-41829-y. 10.1038/s41467-023-41829-y PubMed 37794046