pcDNA TfR1-PuRRRE
(Plasmid
#208327)
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PurposeC-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTfR1-PuRRRE
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- PuRRRE (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA TfR1-PuRRRE was a gift from Kimberly Beatty (Addgene plasmid # 208327 ; http://n2t.net/addgene:208327 ; RRID:Addgene_208327) -
For your References section:
Orthogonal Versatile Interacting Peptide Tags for Imaging Cellular Proteins. Suyama A, Devlin KL, Macias-Contreras M, Doh JK, Shinde U, Beatty KE. Biochemistry. 2023 Jun 6;62(11):1735-1743. doi: 10.1021/acs.biochem.2c00712. Epub 2023 May 11. 10.1021/acs.biochem.2c00712 PubMed 37167569