pET28b-PuRRRE
(Plasmid
#208323)
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PurposeBacterial expression of the His-tagged peptide PuRRRE.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5368
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUse BL21-DE3* for expression
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePuRRRE
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Alt namePuRRRE-DBCO
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SpeciesSynthetic
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Tag
/ Fusion Protein
- DBCO-Tag, His-Tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7-F
- 3′ sequencing primer T7 REV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b-PuRRRE was a gift from Kimberly Beatty (Addgene plasmid # 208323 ; http://n2t.net/addgene:208323 ; RRID:Addgene_208323) -
For your References section:
Orthogonal Versatile Interacting Peptide Tags for Imaging Cellular Proteins. Suyama A, Devlin KL, Macias-Contreras M, Doh JK, Shinde U, Beatty KE. Biochemistry. 2023 Jun 6;62(11):1735-1743. doi: 10.1021/acs.biochem.2c00712. Epub 2023 May 11. 10.1021/acs.biochem.2c00712 PubMed 37167569