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pET28b-PunyEEER
(Plasmid #208322)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208322 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB5alpha
  • Growth instructions
    Use BL21-DE3* for expression
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PunyERRE
  • Alt name
    PunyERRE-DBCO
  • Alt name
    Puny
  • Species
    Synthetic
  • Tag / Fusion Protein
    • DBCO-Tag, His-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7-F
  • 3′ sequencing primer T7 REV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-PunyEEER was a gift from Kimberly Beatty (Addgene plasmid # 208322 ; http://n2t.net/addgene:208322 ; RRID:Addgene_208322)

  • For your References section:

    Orthogonal Versatile Interacting Peptide Tags for Imaging Cellular Proteins. Suyama A, Devlin KL, Macias-Contreras M, Doh JK, Shinde U, Beatty KE. Biochemistry. 2023 Jun 6;62(11):1735-1743. doi: 10.1021/acs.biochem.2c00712. Epub 2023 May 11. 10.1021/acs.biochem.2c00712 PubMed 37167569
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