pET28a-6xHis-MBP-TEV site-TadA8e
(Plasmid
#208319)
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PurposeOverxpression MBP-TadA8e
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208319 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5201
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTadA8e
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SpeciesE. coli
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Insert Size (bp)501
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Mutationmutations in TadA are described in the paper
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-6xHis-MBP-TEV site-TadA8e was a gift from Weixin Tang (Addgene plasmid # 208319 ; http://n2t.net/addgene:208319 ; RRID:Addgene_208319) -
For your References section:
An adenine base editor variant expands context compatibility. Xiao YL, Wu Y, Tang W. Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01994-3. 10.1038/s41587-023-01994-3 PubMed 38168987