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Addgene

ABE-RA1.0
(Plasmid #208297)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208297 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with a BR322 origin
  • Backbone size w/o insert (bp) 3402
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TadA-RA1.0-SpCas9 D10A
  • Species
    E. coli
  • Insert Size (bp)
    4818
  • Mutation
    D108G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ABE-RA1.0 was a gift from Weixin Tang (Addgene plasmid # 208297 ; http://n2t.net/addgene:208297 ; RRID:Addgene_208297)

  • For your References section:

    An adenine base editor variant expands context compatibility. Xiao YL, Wu Y, Tang W. Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01994-3. 10.1038/s41587-023-01994-3 PubMed 38168987
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