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OptoProfilin.S138A
(Plasmid #208288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4018
  • Total vector size (bp) 6790
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cry2 - mCherry - Profilin
  • Alt name
    profilin-1
  • Species
    M. musculus (mouse), A. thaliana (mustard weed)
  • Insert Size (bp)
    2772
  • Mutation
    Ser 138 (sometimes numbered 137) mutated to Ala
  • Entrez Gene
    Cry2 (a.k.a. D130054K12Rik)
  • Entrez Gene
    PRF1 (a.k.a. AT2G19760, F6F22.21, F6F22_21, PFN1, PROFILIN 1, profilin 1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer pSV40A Reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.04.560945 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OptoProfilin.S138A was a gift from Robert Hughes (Addgene plasmid # 208288 ; http://n2t.net/addgene:208288 ; RRID:Addgene_208288)

  • For your References section:

    OptoProfilin: A Single Component Biosensor of Applied Cellular Stress. Mann N., Hill J., Wang K., & Hughes R.M.. ChemBioChem, 8 March 2024 10.1002/cbic.202400007
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