pLRD13
(Plasmid
#208265)
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PurposeProduction of ent-cassa-12,15-diene in E. coli MEV20
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLRD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nametHMGR
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SpeciesSaccharomyces cerevisiae
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Gene/Insert 2
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Gene/Insert nameptmT2
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SpeciesStreptomyces platensis
Gene/Insert 3
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Gene/Insert nameosKSL7
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SpeciesOryza sativa subsp. Japonica
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLRD13 was a gift from Christopher Voigt (Addgene plasmid # 208265 ; http://n2t.net/addgene:208265 ; RRID:Addgene_208265) -
For your References section:
Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s. Lin GM, Voigt CA. Nature Catalysis (2023) 10.1038/s41929-023-01049-5