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pSC101-GFPmut2-mScarlet-I T7
(Plasmid #208191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Backbone size w/o insert (bp) 3968
  • Total vector size (bp) 5626
  • Vector type
    Bacterial Expression ; reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    GFPmut2
  • Species
    Synthetic
  • Insert Size (bp)
    725
  • GenBank ID
  • Promoter Ptet+dnaK P1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mScarlet-I
  • Species
    Synthetic
  • Insert Size (bp)
    702
  • Mutation
    Frameshift insertion 6TTTTTTT
  • GenBank ID
  • Promoter Ptet+dnaK P1

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSC101-GFPmut2-mScarlet-I T7 was a gift from Tanel Tenson (Addgene plasmid # 208191 ; http://n2t.net/addgene:208191 ; RRID:Addgene_208191)

  • For your References section:

    Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation. Hinnu M, Putrins M, Kogermann K, Kaldalu N, Tenson T. Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8. 10.1038/s41598-024-57597-8 PubMed 38519558
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