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SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala
(Plasmid #208173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
  • Alt name
    Yeast αWD40 Arg300Ala
  • Species
    S. pombe (fission yeast)
  • Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys, Arg300Ala
  • GenBank ID
    2541412
  • Tag / Fusion Protein
    • Strep-tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala was a gift from Syed Saif Hasan (Addgene plasmid # 208173 ; http://n2t.net/addgene:208173 ; RRID:Addgene_208173)

  • For your References section:

    A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs. Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher T, Hasan SS. Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3. 10.1038/s41467-023-44076-3 PubMed 38102143
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