SSH-pSUMO-β′-COPI-WD40-Tyr33Ala
(Plasmid
#208170)
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PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
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Alt nameYeast β′WD40 Y33A
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SpeciesS. cerevisiae (budding yeast)
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GenBank IDU11237
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Tags
/ Fusion Proteins
- Strep-tag (N terminal on backbone)
- His-tag (N terminal on backbone)
- SUMO-tag (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SSH-pSUMO-β′-COPI-WD40-Tyr33Ala was a gift from Syed Saif Hasan (Addgene plasmid # 208170 ; http://n2t.net/addgene:208170 ; RRID:Addgene_208170) -
For your References section:
A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs. Dey D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban J, Gallagher T, Hasan SS. Nat Commun. 2023 Dec 15;14(1):8358. doi: 10.1038/s41467-023-44076-3. 10.1038/s41467-023-44076-3 PubMed 38102143