pGSTag Flag p38 alpha (agf)
(Plasmid #20816)

• Depositing Lab: Roger Davis
• Publication: Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Backbone

• Vector backbone: pGSTag
• Backbone size w/o insert (bp): 5057
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pGSTag Flag p38 alpha (agf)
• Alt name: MapK14
• Species: M. musculus (mouse)
• Insert Size (bp): 1135
• Mutation: Replace Thr 180 and Tyr 182 with Ala and Phe respectivly
• GenBank ID: NM_011951
• Entrez Gene: Mapk14 (a.k.a. CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: xba I (not destroyed)
• 5′ sequencing primer: pGex 5'
• 3′ sequencing primer: pGex 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: original wild-type p38 alpha insert provided by Jiahuai Han and Richard J. Ulevitch, "A Map Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian Cells", (Science,vol.265, pg806)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry