CN2109-rAAV-eHGT_410m-minBglobin-SYFP2-WPRE3-BGHpA
(Plasmid
#208157)
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PurposeDirect-expressing SYFP2 in oligodendrocytes AAV Virus
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonerAAV
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Backbone manufacturerAllen Institute for Brain Science
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYFP2
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SpeciesSynthetic
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Insert Size (bp)720
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MutationNA
- Promoter Beta Globin minimal promoter
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Funded by the Brain Initiative. Please visit https://doi.org/10.1101/2023.09.20.558718 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CN2109-rAAV-eHGT_410m-minBglobin-SYFP2-WPRE3-BGHpA was a gift from The Allen Institute for Brain Science & Boaz Levi (Addgene plasmid # 208157 ; http://n2t.net/addgene:208157 ; RRID:Addgene_208157) -
For your References section:
Enhancer-AAVs allow genetic access to oligodendrocytes and diverse populations of astrocytes across species. Mich JK, Sunil S, Johansen N, Martinez RA, Leytze M, Gore BB, Mahoney JT, Ben-Simon Y, Bishaw Y, Brouner K, Campos J, Canfield R, Casper T, Dee N, Egdorf T, Gary A, Gibson S, Goldy J, Groce EL, Hirschstein D, Loftus L, Lusk N, Malone J, Martin NX, Monet D, Omstead V, Opitz-Araya X, Oster A, Pom CA, Potekhina L, Reding M, Rimorin C, Ruiz A, Sedeño-Cortés AE, Shapovalova NV, Taormina M, Taskin N, Tieu M, Valera Cuevas NJ, Weed N, Way S, Yao Z, McMillen DA, Kunst M, McGraw M, Thyagarajan B, Waters J, Bakken TE, Yao S, Smith KA, Svoboda K, Podgorski K, Kojima Y, Horwitz GD, Zeng H, Daigle TL, Lein ES, Tasic B, Ting JT, Levi BP. bioRxiv 2023.09.20.558718 10.1101/2023.09.20.558718