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Addgene

pGSTag Flag p38 alpha
(Plasmid #20815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGSTag
  • Backbone size w/o insert (bp) 5057
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pGSTag Flag p38 alpha
  • Alt name
    MapK14
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1135
  • GenBank ID
    NM_011951
  • Entrez Gene
    Mapk14 (a.k.a. CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site xba I (not destroyed)
  • 5′ sequencing primer pGex 5'
  • 3′ sequencing primer pGex 3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original wild-type p38 alpha insert provided by Jiahuai Han and Richard J. Ulevitch, "A Map Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian Cells", (Science,vol.265, pg806)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGSTag Flag p38 alpha was a gift from Roger Davis (Addgene plasmid # 20815 ; http://n2t.net/addgene:20815 ; RRID:Addgene_20815)

  • For your References section:

    Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. Enslen H, Raingeaud J, Davis RJ. J Biol Chem. 1998 Jan 16. 273(3):1741-8. 10.1074/jbc.273.3.1741 PubMed 9430721
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