Skip to main content
Addgene

pWZ699
(Plasmid #208117)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    F plasmid
  • Backbone manufacturer
    N/A
  • Backbone size (bp) 13000
  • Modifications to backbone
    Inserting yeast replication origin, auxotrophic marker LEU2, mouse Actb gene, and thymidine kinase transcription unit.
  • Vector type
    Mammalian Expression, Bacterial Expression, Yeast Expression, Synthetic Biology ; Copy number inducible
  • Promoter Human PGK1 for thymidine kinase
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    EPi300
  • Growth instructions
    Adding 0.2% arabinose for copy number induction.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZ699 was a gift from Jef Boeke (Addgene plasmid # 208117 ; http://n2t.net/addgene:208117 ; RRID:Addgene_208117)

  • For your References section:

    Mouse genome rewriting and tailoring of three important disease loci. Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM, Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ, Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD. Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4. 10.1038/s41586-023-06675-4 PubMed 37914927
Related items:
Commonly requested with: