pWZ854
(Plasmid #208116)

• Purpose: mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo
• Depositing Lab: Jef Boeke
• Publication: Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4.

Backbone

• Vector backbone: TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 8027
• Modifications to backbone: No
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeonGreen-HPRT1-Neo
• Alt name: Marker cassette 2.3
• Species: H. sapiens (human)
• Insert Size (bp): 4500
• GenBank ID: NM_000194.3
• Promoter: EF1alpha
• Tag / Fusion Protein:
  • T2A (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pWZ854_MC2.3_Topo.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry