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pWZ853
(Plasmid #208115)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208115 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 7616
  • Modifications to backbone
    No
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen-HPRT1-BSD
  • Alt name
    Marker cassette 2.2
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    4100
  • GenBank ID
    NM_000194.3
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • T2A (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZ853 was a gift from Jef Boeke (Addgene plasmid # 208115 ; http://n2t.net/addgene:208115 ; RRID:Addgene_208115)

  • For your References section:

    Mouse genome rewriting and tailoring of three important disease loci. Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM, Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ, Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD. Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4. 10.1038/s41586-023-06675-4 PubMed 37914927
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