pWZ852
(Plasmid
#208114)
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PurposemSwAP-In marker cassette 1 with mScarlet-HygTK
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208114 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 7856
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Modifications to backboneNo
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet-HygTK
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Alt nameMarker cassette 1.2
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)4300
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GenBank ID
- Promoter EF1alpha
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Tag
/ Fusion Protein
- T2A (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWZ852 was a gift from Jef Boeke (Addgene plasmid # 208114 ; http://n2t.net/addgene:208114 ; RRID:Addgene_208114) -
For your References section:
Mouse genome rewriting and tailoring of three important disease loci. Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM, Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ, Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD. Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4. 10.1038/s41586-023-06675-4 PubMed 37914927