pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold
(Plasmid #208111)

• Purpose: Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter
• Depositing Lab: Yuxuan Guo
• Publication: Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. doi: 10.1111/jcmm.18145.

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Backbone

• Vector backbone: pAAV-CASI-Cas9C
• Backbone size w/o insert (bp): 6849
• Total vector size (bp): 7245
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpG C, U6, scaffold
• Alt name: Split-SpG C-terminal half and scaffold
• Species: Synthetic
• Insert Size (bp): 2587

Resource Information

• Supplemental Documents:
  • paav-casi-inteinc-aa713-spg-c-u6-aari-grna-inverted-pd076.gb
• A portion of this plasmid was derived from a plasmid made by: addgene 140002:pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562);addgene 132778:pU6-Sp-pegRNA-HEK3_CTT_ins

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry