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pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1
(Plasmid #208110)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208110 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CASI-Cas9C
  • Backbone size w/o insert (bp) 6849
  • Total vector size (bp) 7245
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpG C, U6, Lmna sgRNA1, scaffold
  • Alt name
    Split-SpG C-terminal half and Lmna sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    2578
  • Promoter U6

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    addgene 140002:pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562);addgene 132778:pU6-Sp-pegRNA-HEK3_CTT_ins

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1 was a gift from Yuxuan Guo (Addgene plasmid # 208110 ; http://n2t.net/addgene:208110 ; RRID:Addgene_208110)

  • For your References section:

    Adenine base editor-based correction of the cardiac pathogenic Lmna c.1621C > T mutation in murine hearts. Yang L, Liu Z, Sun J, Chen Z, Gao F, Guo Y. J Cell Mol Med. 2024 Feb;28(4):e18145. doi: 10.1111/jcmm.18145. 10.1111/jcmm.18145 PubMed 38332517
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