pRSF- RPA4 (MSW#716)
(Plasmid
#208077)
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PurposeExpression of human Replication Protein A 4 in E.coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSFDuet-1
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Backbone manufacturerMilliporeSigma (Novagen)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPA4
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SpeciesH. sapiens (human)
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Entrez GeneRPA4 (a.k.a. HSU24186)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
RPA4 gene under the control of a T7 RNA polymerase promoter. Induction with IPTG expresses RPA4.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSF- RPA4 (MSW#716) was a gift from Marc Wold (Addgene plasmid # 208077 ; http://n2t.net/addgene:208077 ; RRID:Addgene_208077) -
For your References section:
An alternative form of replication protein a prevents viral replication in vitro. Mason AC, Haring SJ, Pryor JM, Staloch CA, Gan TF, Wold MS. J Biol Chem. 2009 Feb 20;284(8):5324-31. doi: 10.1074/jbc.M808963200. Epub 2008 Dec 29. 10.1074/jbc.M808963200 PubMed 19116208
