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pET16b-RPA4/His14 (MSW#949)
(Plasmid #208075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16b
  • Backbone manufacturer
    MilliporeSigma (Novagen)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA4, RPA14(His-tagged)
  • Alt name
    Gene ID: 29935, 6119
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    -1
  • Tag / Fusion Protein
    • 10xHis tag on RPA14 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains a single T7 RNA polymerase promoter followed by RPA4 and His-tagged RPA14. (Each gene has Shine-Delgarno ribosome binding site.) Induction with IPTG expresses RPA14 and RPA4 which form a soluble subcomplex.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET16b-RPA4/His14 (MSW#949) was a gift from Marc Wold (Addgene plasmid # 208075 ; http://n2t.net/addgene:208075 ; RRID:Addgene_208075)

  • For your References section:

    An alternative form of replication protein a prevents viral replication in vitro. Mason AC, Haring SJ, Pryor JM, Staloch CA, Gan TF, Wold MS. J Biol Chem. 2009 Feb 20;284(8):5324-31. doi: 10.1074/jbc.M808963200. Epub 2008 Dec 29. 10.1074/jbc.M808963200 PubMed 19116208
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