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pEGFP-hsRPA32-Asp8 (MSW#507)
(Plasmid #208072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA2
  • Alt name
    RPA32
  • Species
    H. sapiens (human)
  • Mutation
    mutations of 8 ser and thr residues in N-term domain: S8,11-13D,S23,29, 33D; T21D
  • Entrez Gene
    RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Quick-change mutations (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid to express GFP-hsRPA32 mutant in human cells. hsRPA32-Asp8 gene from PMID: 12819197, subcloned into pEGFP as in PMID: 19942684.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hsRPA32-Asp8 (MSW#507) was a gift from Marc Wold (Addgene plasmid # 208072 ; http://n2t.net/addgene:208072 ; RRID:Addgene_208072)
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