pEGFP-hsRPA4 (MSW#517)
(Plasmid
#208070)
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PurposeExpression of GFP-tagged hsRPA4 in human cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRPA4
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SpeciesH. sapiens (human)
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Entrez GeneRPA4 (a.k.a. HSU24186)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-hsRPA4 (MSW#517) was a gift from Marc Wold (Addgene plasmid # 208070 ; http://n2t.net/addgene:208070 ; RRID:Addgene_208070) -
For your References section:
A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression. Haring SJ, Humphreys TD, Wold MS. Nucleic Acids Res. 2010 Jan;38(3):846-58. doi: 10.1093/nar/gkp1062. Epub 2009 Nov 26. 10.1093/nar/gkp1062 PubMed 19942684