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Addgene

pEGFP-hsRPA4 (MSW#517)
(Plasmid #208070)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208070 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA4
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPA4 (a.k.a. HSU24186)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hsRPA4 (MSW#517) was a gift from Marc Wold (Addgene plasmid # 208070 ; http://n2t.net/addgene:208070 ; RRID:Addgene_208070)
  • For your References section:

    A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression. Haring SJ, Humphreys TD, Wold MS. Nucleic Acids Res. 2010 Jan;38(3):846-58. doi: 10.1093/nar/gkp1062. Epub 2009 Nov 26. 10.1093/nar/gkp1062 PubMed 19942684